Raman Spectroscopy is a very powerful technique able to extract information from the chemical bonds of a material. Its spectral signal is very characteristic and has been used as an analytic tool in multiple applications like material science, chemistry and others. Its advances especially in microscopy and optics have enabled its application in molecular biology. In particular, Raman Spectroscopy provides a spectral fingerprint from the set of bonds that are present in the material under examination. The spectral frequencies contain a complex mixture of details deriving from the structure and local environment of the molecules. The major advantage of Raman spectroscopy is that it is simple, label free, reproducible, non-destructive, requires only small amounts of sample, and minimum sample preparation. Its applications in biology and medicine are multiple and growing rapidly, including but not limited to the analysis of proteins, lipids, DNA, live- and fixed- cell, Pharmacokinetics, and tissue imaging.
Part of this work is supported by the project “Advanced Research Activities in Biomedical and Agro alimentary Technologies” (MIS 5002469) which is implemented under the “Action for the Strategic Development on the Research and Technological Sector”, funded by the Operational Programme "Competitiveness, Entrepreneurship and Innovation" (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund).