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| Description: | InnovCrete Seminar NMR Insights on the conformational plasticity and interaction of biomolecules Prof. George Spyroulias, Patras University, GREECE Thursday, April 21st, 2016 @ 13:00 FORTH Amphitheatre Host: M. Kokkinidis The use of Modern Biomolecular NMR which is a powerful technique for the investigation of the conformational dynamics and structure-activity correlation of proteins and their complexes, provides atomic-level insights about the conformational dynamics of these biologicaly relevant molcules. To achieve this goal it is required the concerted action of molecular biology, spectroscopy and computational biology for the high-yield production of proteins in suitable forms for NMR studies (uniform labeling in 2H, 13C, 15N nuclei, and specific labeling of residues or chemical groups in 13C, 15N), the acquisition a series of NMR data and the determination of the 3D structures in solution along with the ellucidation of the relaxation properties of proteins with molecular weight ranging from 7 to >25 kDa. Among our current research interests is the NMR-driven conformational dynamics study of disease-related enzymes and proteins, such as: (i) RNA binding proteins (~350 aminoacids) [1-2], which are key-factors in proper folding of pre-tRNA transcripts, like La autoantigen (ii) Heme-nitric oxide/oxygen binding (H-NOX) motifs (~200 amino acids), which exist as proteins or domains within larger proteins, such as soluble guanylate cyclase and function as a sensor for the gaseous signaling agent nitric oxide (NO) that regulates the catalytic site contained within the C-terminal end of the enzyme [3], (iii) macro domains (~170 aminoacids), which are evolutionarily conserved in eukaryotic organisms, bacteria and archaea and they are also found in nonstructural proteins (nsPs) of several positive-strand RNA viruses, including hepatitis E virus, rubella virus and coronaviruses, as well as alphaviruses [4-5], (iv) various E3 ubiquitin ligases involved in ubiquitination pathway, like ARKADIA tumor suppressor [6-7], (v) extracellular domains of ligand-gated ion channels of the cys-loop family (nAChR is the prototype) that involved in various neurodegenerative disorders [8], and others. |
| Location: | FORTH Amphitheatre |
| Status: | Waiting for approval |
| Date: | Thursday, April 21, 2016 |
| Time: | 13:00 EEST |
| Priority: | 5-Medium |
| Access: | Public |
| Created by: | Lila Kalogeraki |
| Updated: | Friday, April 15, 2016 12:52 GMT |