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[11:00] IMBB Seminar
- Description:
- Dr. Imre Berger
EMBL Group Leader, Grenoble Outstation
Head, Eukaryotic Expression Facility (EEF)
"MultiBac: Expanding the research tool-box for multiprotein complexes"
Monday, 13 May 2013 @ 11.00
Seminar Room No 1, 1st floor FORTH's bldg
Host: Dr. Thanasis Loukeris
Abstract:
Multiprotein complexes catalyze vital biological functions in the cell. A paramount objective of current research efforts is to address the structural molecular biology of these molecular machines, by enlisting and developing enabling technologies for their study. An emerging key prerequisite for studying complex biological specimens is their recombinant overproduction. We have designed and validated novel reagents and streamlined protocols for rapidly assembling
co-expression constructs for this purpose. The ACEMBL high-throughput pipeline for multiprotein complexes at the EMBL Grenoble is presented.
Heterologous expression of eukaryotic proteins and their complexes increasingly necessitate eukaryotic overproduction systems. Extension of the ACEMBL platform technology to eukaryotic hosts, insect cells and mammalian, has been achieved. Efficient production of large multicomponent protein complexes for structural studies using the baculovirus/insect cell system can be hampered by stoichiometric imbalance of the subunits produced. A polyprotein strategy has been developed to overcome this bottleneck and successfully implemented in our MultiBac baculovirus expression system for producing multiprotein complexes. Our technologies and their successful application to academic and industrial R&D projects, are discussed. The EC FP7
projects ComplexINC and BioSTRUCT-X for accessing our platform will be introduced.
http://www.embl.fr/research/unit/berger/members/?s_personId=CP-60003124
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May 2013
