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| 10:00 |
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| 11:00 |
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| 12:00 |
[12:00] InnovCrete Seminar
- Description:
- InnovCrete Seminar
How do proteins evolve?
Prof. Dan S. Tawfik, Weizmann Institute of Science, Israel
Friday, April 22nd, 2016 @ 12:00
FORTH Amphitheatre
Host: M. Kokkinidis
I will briefly describe our insights regarding how new enzymatic functions evolve, via gradual, small changes in sequence, and while preserving the overall structure (fold) and active-site architecture. However, the mechanisms underlying these evolutionary micro-transitions do not explain the macro evolutionary transitions – how completely new folds emerge, let alone how the very first protein(s) evolve. The latter are thought to have emerged from short polypeptides. I will describe our findings with respect to how functional, globular proteins may emerge from short peptides.
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| 13:00 |
[13:00] InnovCrete Seminar
- Description:
- InnovCrete Seminar
Drug Design via XRPD: From a protein microcrystalline sample to experimental powder diffraction data and the derived structural model
Prof. Irene Margiolaki, Patras University, GREECE
Friday, April 22nd, 2016 @ 13:00
FORTH Amphitheatre
Host: M. Kokkinidis
The development of the X-ray powder diffraction (XRPD) methods and algorithms, has already allowed for the structural characterization of a range of proteins and has been established by our group as a useful complementary technique to the traditional single crystal diffraction techniques. Protein polycrystalline samples consist of a large number of randomly oriented microcrystals. They often form in a short period of time and under different conditions of physico - chemical environment. To date, our studies on Urate Oxidase and complexes of human insulin with organic ligands, revealed a high degree of polymorphism, as well as crystalline polymorphs which were not previously identified probably due to the lack of adequate tools to characterize microcrystalline protein precipitates. The aim of this talk is to describe the frontier of powder diffraction as it begins to make a significant impact on structural biology. Issues which will be discussed include: (a) application of the molecular replacement technique and structure refinements of selected proteins associated with pharmaceutical interest (b) high throughput investigations such as crystal screening and phase diagram mapping and (c) ligand binding.
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| 14:00 |
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| 15:00 |
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| 16:00 |
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| 21:00 |
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April 2016
